This 2024 annual report is the result of coordinated work between the National Institute of Toxicology and Forensic Sciences (INTCF), the Institute of Legal Medicine and Forensic Sciences of Catalonia (IMLCFC), the Institute of Legal Medicine of the Basque Country (IVML), the Institute of Legal Medicine and Forensic Sciences of Aragon (IMLCFA), the Institute of Legal Medicine and Forensic Sciences of Murcia (IMLCFM), the Institute of Legal Medicine and Forensic Sciences of Valencia (IMLCFV), the Institute of Legal Medicine and Forensic Sciences of the Balearic Islands (IMLCFIB) and the Luis Concheiro Forensic Sciences Institute (INCIFOR), in collaboration with the National Road Safety Observatory (ONSV) of the General Directorate of Traffic (DGT). It is aimed at improving knowledge of traffic fatalities during 2024 that were investigated from a toxicological-forensic perspective throughout the national territory.